论文部分内容阅读
目的 :探讨碱烧伤后角膜内抗氧化酶 ,即超氧化物歧化酶 (SOD) ,过氧化氢酶 (CAT) ,谷胱甘肽过氧化酶 (GPx)的mRNA的表达的动态变化 ,从而进一步揭示角膜碱烧伤的损伤机制。方法 :根据Roper Hall分度法[1] ,分别制造轻、重两组碱烧伤模型 ,采用RT PCR的方法研究碱烧伤后大鼠角膜内抗氧化酶的mRNA表达的变化。结果 :在轻致伤组中 ,超氧化物歧化酶在伤后 2周时的mRNA表达下降与前期有明显差异 (P <0 0 5 )。过氧化氢酶在伤后 2天时其mRNA表达升至最高点 ,2周时明显下降。谷胱甘肽过氧化酶伤后 10分钟至 30分钟其mRNA的表达即为高水平。于 2天时 ,降至最低点。 1周后回升至正常。 2周时再次明显下降。在重致伤组中 ,超氧化物歧化酶在伤后 10分钟与烧伤后 2周其mRNA的表达明显下降 ,过氧化氢酶在伤后 10分钟就开始下降 ,30分钟至 6 0分钟达极低水平 ,2周时再次达低水平。谷胱甘肽过氧化酶在伤后 6 0分钟及2周时达最低水平。以上变化均具有显著性差异。结论 :角膜碱烧伤后 ,角膜内抗氧化酶的mRNA表达 ,并不是一味的下降 ,说明机体基因水平具有很高的应激能力。这对临床碱烧伤的治疗将会提供新的帮助
Objective: To investigate the dynamic changes of intracellular corneal anti-oxidative enzymes (SOD), catalase (CAT) and glutathione peroxidase (GPx) mRNA expression after alkali burns, Reveal the corneal alkali burn injury mechanism. Methods: According to Roper Hall indexing method [1], the models of alkali burn in both groups were made respectively. The changes of mRNA expression of antioxidant enzymes in the cornea of alkali burn rats were detected by RT-PCR. Results: The mRNA expression of superoxide dismutase (SOD) in the injured group was significantly decreased at 2 weeks after injury (P <0 05). At 2 days after injury, catalase increased its mRNA expression to the highest point and decreased significantly at 2 weeks. Glutathione peroxidase 10 to 30 minutes after injury its mRNA expression is high. In 2 days, to the lowest point. Return to normal after 1 week. 2 weeks significantly decreased again. In the severe injury group, the expression of superoxide dismutase decreased significantly at 10 minutes after injury and 2 weeks after burn, and catalase began to decrease 10 minutes after injury, up to 30 minutes to 60 minutes Low level, once again reached a low level at 2 weeks. Glutathione peroxidase at 60 minutes and 2 weeks after injury reached the lowest level. The above changes have significant differences. CONCLUSION: After corneal alkali burn, the mRNA expression of anti-oxidase in the cornea does not decrease blindly, indicating that the gene level of the body has a high stress ability. This treatment of clinical alkali burn will provide new help