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以抗感大豆胞囊线虫3号生理小种的小粒黑豆(ZDD1412)和辽豆10为试材,温室盆栽条件下人工接种大豆胞囊线虫,以未接种作对照,接种后7、14、21、28和35 d取样,测定大豆根内抗坏血酸含量和抗坏血酸过氧化物酶(APX)活性的动态变化以及不同浓度抗坏血酸对大豆胞囊线虫胞囊孵化和二龄幼虫抑制率的影响。结果表明:抗病品种抗坏血酸含量大部分时间高于感病品种,接种试材抗坏血酸含量的变化幅度大于未接种对照;抗病品种APX活性均呈上升趋势,而感病品种接种和未接种的APX活性变化幅度较大。浓度为5、10和20 mg.L-1的抗坏血酸对胞囊孵化的刺激效果最为显著;浓度为80 mg.L-1的抗坏血酸对二龄幼虫的校正击倒率在不同处理时间上均大于其它浓度处理,均与无菌水对照达到了5%的显著水平。
In this study, soybean cyst nematode was inoculated artificially in greenhouse under the conditions of non-inoculation as control, 7,14,21 days after inoculation with ZDD1412 and Liao Dou 10, 28 and 35 d, the dynamic changes of ascorbate content and ascorbate peroxidase (APX) activity in soybean roots and the effects of different concentrations of ascorbic acid on the cyst hatching and second-instar larvae inhibition of soybean cyst nematode were determined. The results showed that the content of ascorbic acid in resistant cultivars was higher than susceptible cultivars most of the time, and the variation of ascorbic acid content in test inoculated plants was greater than that in non-inoculated plants. APX activity of resistant cultivars showed an upward trend. However, APX Changes in the activity range. Ascorbic acid at concentrations of 5, 10 and 20 mg.L-1 had the most obvious stimulating effect on the cyst hatching; the correction knock-out rate of ascorbic acid at the concentration of 80 mg.L-1 for second-instar larvae was greater than that of the second- Other concentrations of treatment, both with sterile water control reached 5% of the significant level.