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目的:研究Runx3基因CpG岛甲基化对胃癌病变的影响;探讨甲基化特异性聚合酶链法(MSP)和焦磷酸测序法(PS)对Runx3基因甲基化检测的特异性。方法:选取150例胃癌患者胃切除标本及50例无癌变胃黏膜标本,以MSP法和PS法同时检测其Runx3启动子的甲基化程度。结果:与正常胃黏膜相比,胃癌组织Runx3的甲基化程度差异有统计学意义(MSP法:67.3%vs 40.0%;PS法:76.0%vs 30.0%)。MSP结果显示,Runx3甲基化只与肿瘤大小相关,P<0.05。而PS法分析显示进展期胃癌的Runx3甲基化率高于早期胃癌;且其甲基化状态与肿瘤的大小(P=0.001)、Lauren分级(P=0.043)、浸润的深度(P<0.001)、淋巴结转移(P=0.004)及肿瘤TNM临床分期(P=0.003)等临床病理特征之间存在相关性。结论:Runx3启动子甲基化与胃癌临床病理特征密切相关,对于胃癌的早期诊断和治疗有重要意义。
OBJECTIVE: To study the effect of Runx3 CpG island methylation on the pathological changes of gastric cancer and to investigate the specificity of methylation-specific polymerase chain reaction (MSP) and pyrosequencing (PS) on Runx3 methylation. Methods: 150 cases of gastric cancer patients with gastric resection and 50 cases of non-cancerous gastric mucosa specimens were selected to detect the degree of methylation of Runx3 promoter by MSP method and PS method. Results: Compared with normal gastric mucosa, the methylation degree of Runx3 in gastric cancer tissues was statistically significant (MSP method: 67.3% vs 40.0%; PS method: 76.0% vs 30.0%). MSP results showed that Runx3 methylation only correlated with tumor size, P <0.05. However, the methylation status of Runx3 in advanced gastric cancer was higher than that in early stage gastric cancer by PS method. The methylation status was associated with tumor size (P = 0.001), Lauren grade (P = 0.043), depth of invasion ), Lymph node metastasis (P = 0.004) and clinical TNM stage (P = 0.003). Conclusion: Runx3 promoter methylation is closely related to the clinicopathological features of gastric cancer, which is of great significance for the early diagnosis and treatment of gastric cancer.