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目的评价以血浆1,3-β-D-葡聚糖检测来诊断糖尿病急性并发症合并深部真菌感染的价值。方法以G试验方法定量检测糖尿病急性并发症合可疑并深部真菌感染的患者血浆1,3-β-D-葡聚糖浓度;比较其与真菌培养法的差异。结果 50例可疑深部真菌感染糖尿病急性并发症患者,经真菌培养阳性结果 26例,阳性率为52.0%;血浆1,3-β-D-葡聚糖测法阳性35例,阳性率70.0%,血浆1,3-β-D-葡聚糖检测法阳性率明显高于真菌培养法(χ2=7.11,P<0.05)。深部真菌感染培养阳性组血浆1,3-β-D-葡聚糖浓度为(83.64士31.85)ng/l,健康对照组浓度为(5.097±0.2548)ng/l,深部真菌感染组血浆1,3-β-D-葡聚糖浓度明显高于正常对照组(P<0.001)。结论利用G试验定量检测血浆1,3-β-D-葡聚糖,方法简便,结果稳定,阳性率高,可用于糖尿病急性并发症合并深部真菌感染的早期快速诊断。
Objective To evaluate the value of detecting plasma 1,3-β-D-glucan to diagnose acute complications of diabetes complicated with deep fungal infection. Methods The plasma concentration of 1,3-β-D-glucan in patients with acute diabetic complications and deep fungal infection was quantitatively determined by G test. The difference between the two methods was compared with that of fungal culture. Results In 50 patients with suspected deep fungal infection of acute complications of diabetes, 26 were positive for fungal culture, the positive rate was 52.0%. The positive rate of plasma 1,3-β-D-glucan was 35%, the positive rate was 70.0% The positive rate of plasma 1,3-β-D-glucan was significantly higher than that of fungal culture (χ2 = 7.11, P <0.05). The concentration of 1,3-β-D-glucan in the group with positive fungal infection was (83.64 ± 31.85) ng / l, that of the healthy control group was (5.097 ± 0.2548) ng / l, 3-β-D-glucan concentration was significantly higher than the normal control group (P <0.001). Conclusions G-test can quantitatively detect plasma 1,3-β-D-glucan. The method is simple, stable and has a high positive rate. It can be used for the early rapid diagnosis of acute complications of diabetes mellitus complicated with deep fungal infection.