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探讨肿瘤坏死因子(TNF_α)在全身炎症反应综合征(SIRS)发病中的作用。用双抗体夹心酶联免疫吸附(ELISA)法测定51例中枢感染患儿(其中SIRS23例,非SIRS 28例)和29例正常健康儿童的血清TNF_α含量。结果3组患儿血清TNF_α含量的几何均数分别为SIRS组386.81(范围227.38～906.90)、非SIRS组277.59(范围186.61～420.51)、对照组80.88(范围34.52～190.59)ng/L。SIRS组患儿血清TNF_α含量明显高于其它两组(P均<0.01),且符合SIRS诊断标准项目越多,血清TNF_α含量越高。我们认为中枢感染患儿血清TNF_α升高与SIRS的发生发展有关。
To investigate the role of tumor necrosis factor (TNF_α) in the pathogenesis of systemic inflammatory response syndrome (SIRS). Serum TNF-α levels in 51 children with central infection (23 SIRS cases, 28 non-SIRS) and 29 normal healthy children were determined by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). Results The geometric mean TNFα levels in three groups were 386.81 (range 227.38-906.90) in SIRS group, 277.59 (range 186.61-420.51) in non-SIRS group and 80.88 (range 34.52-190.59) ng / L in control group respectively. The levels of serum TNF-α in SIRS group were significantly higher than those in the other two groups (all P <0.01). The more the items meeting SIRS diagnostic criteria, the higher the serum TNF-α level. We think that the elevated serum TNF-α in children with central infection is related to the occurrence and development of SIRS.