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目的:建立鬼箭羽总黄酮的含量测定方法并筛选其提取方法。方法:以芦丁为对照品,通过单因素试验优选显色方法和显色条件,建立总黄酮含量测定的方法学考察并优选其提取方法。结果:采用盐酸-镁粉反应比色法,芦丁在0.020~0.104 mg与吸光度呈良好线性关系(r=0.997),平均加样回收率99.424%(RSD 2.220%)。结论:盐酸-镁粉显色法具有精密度高、重复性好等优势,适用于鬼箭羽总黄酮的定量分析。鬼箭羽总黄酮的提取宜采用回流法。
Objective: To establish a method for determination of total flavonoids in ghost arrow feather and to screen its extraction method. Methods: Rutin was used as reference substance, and the method of determination of total flavonoids content and the extraction method were optimized by single factor experiment. Results: The linearity of rutin was 0.020 ~ 0.104 mg (r = 0.997) and the average recovery was 99.424% (RSD 2.220%) using hydrochloric acid - magnesium reaction colorimetry. Conclusion: Hydrochloric acid-magnesium powder colorimetric method has the advantages of high precision and good repeatability, and is suitable for the quantitative analysis of total flavonoids in Ghost Arrows. Ghost arrow feather extraction of flavonoids should be used reflux method.