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目的：探讨中药补肾复方对老年人外周血单核细胞（ＰＢＭＣ）增殖反应及膜流动性的影响。方法：选择老年人肾虚证患者给予补肾复方、治疗前后运用ＭＴＴ比色法检测ＰＢＭＣ对植物血凝素（ＰＨＡ）刺激的增殖反应，同时运用荧光偏振技术以ＤＰＨ（１，６－二苯基，１，３，５－己三烯）为荧光探剂检测ＰＢＭＣ膜流动性。结果：补肾复方可以改善老年人肾虚证症状，并能提高其降低的增殖反应和膜流动性（Ｐ＜０．０５）。结论：补肾复方增强老年人肾虚证患者细胞免疫功能，可能与提高ＰＢＭＣ膜流动性有关
OBJECTIVE: To investigate the effect of compound kidney-tonifying compound recipe on the proliferative response and membrane fluidity of peripheral blood mononuclear cells (PBMC) in the elderly. METHODS: Older patients with kidney-deficiency syndrome were selected and treated with kidney-tonifying compound. Before and after treatment, the proliferative response of PBMC to phytohemagglutinin (PHA) stimulation was measured by MTT colorimetric method. At the same time, fluorescence polarization technique was used to DPH (1,6-diphenyl). 1,3,5-hexatriene was used as a fluorescent probe to detect the membrane fluidity of PBMC. Results: Kidney-tonifying compound can improve the symptoms of kidney deficiency syndrome in elderly people, and can improve its decreased proliferative response and membrane fluidity (P<0.05). Conclusion: Bushen compound can enhance the cellular immune function in elderly patients with kidney deficiency syndrome, which may be related to the increase of PBMC membrane fluidity.