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本实验以人卵巢癌细胞株（ＣＯＣ１）为模型，观察诱导分化剂二甲亚砜（ＤＭＳＯ）与维甲酸（ＲＡ）对该细胞生长增殖、ＤＮＡ合成和转化生长因子β１（ＴＧＦβ１）在细胞内表达的影响。结果显示：ＤＭＳＯ与ＲＡ对人卵巢细胞ＣＯＣ１生长有明显的抑制作用，生长曲线表明作用５天后其生长抑制率分别为６２．７％和４２．１％；３Ｈ－胸腺嘧啶核苷（３Ｈ－ＴｄＲ）掺入实验说明ＤＭＳＯ组与ＲＡ组的单位时间计数率（ＣＰＭ）明显低于对照组（Ｐ＜０．０１）。用药３天后百分掺入抑制率分别为６０．４％与３７．９％，表明ＤＭＳＯ与ＲＡ抑制ＣＯＣ１细胞的ＤＮＡ合成；免疫细胞化学反应表明，ＤＭＳＯ或ＲＡ处理５天后，对照组细胞ＴＧＦβ１表达为阳性，定位于胞浆，而处理组细胞ＴＧＦβ１呈阴性或弱阳性反应。以上结果提示ＤＭＳＯ和ＲＡ对人卵巢癌细胞有一定的诱导分化作用。
In this study, human ovarian cancer cell line (COC1) was used as a model to observe the effects of DMSO and RA on cell proliferation, DNA synthesis and transforming growth factor-β1 (TGFβ1) The impact of expression. The results showed that: DMSO and RA significantly inhibited the growth of human ovarian COC1 cells, and the growth curves showed that the growth inhibition rates were 62.7% and 42.1% after 5 days respectively. The 3H-thymidine (3H-TdR ) Incorporation experiments showed that the unit time count rate (CPM) of DMSO group and RA group was significantly lower than that of the control group (P <0.01). After 3 days of treatment, the percent inhibition was 60.4% and 37.9%, respectively, indicating that DMSO and RA inhibited the DNA synthesis of COC1 cells. Immunocytochemistry showed that after treated with DMSO or RA for 5 days, the expression of TGFβ1 Positive, located in the cytoplasm, while the treatment group cells TGFβ1 was negative or weak positive reaction. The above results suggest that DMSO and RA have some induction and differentiation effects on human ovarian cancer cells.