论文部分内容阅读
目的:研究喉及下咽部鳞状细胞癌组织中血管内皮生长因子C(VEGFC)及其受体VEGFR3(FLT4)的表达情况,以及它们在肿瘤淋巴转移、微转移中的作用和意义。方法:采用免疫组织化学SP法及图像分析技术,检测49例喉及下咽部癌组织中VEGFC、FLT4及淋巴结中VEGFC蛋白表达和相对含量。结果:喉及下咽部癌组织中VEGFC的表达与病理分级密切相关(均P<0.05),与淋巴结转移之间存在相关关系(均P<0.05),与临床T分期无关。FLT4表达与VEGFC阳性表达呈同向性改变,与淋巴结转移相关(均P<0.05);喉及下咽部癌组织中,FLT4阳性脉管数与病理分级及临床T分期差异无统计学意义(均P>0.05)。喉及下咽部癌组织中淋巴结微转移组,VEGFC、FLT4表达与无转移组之间的差异无统计学意义(P>0.05)。结论:VEGFC在喉及下咽部癌组织中,通过其特异性受体FLT4导致淋巴管增生,促进肿瘤淋巴转移,从而影响患者的预后;检测VEGFC、FLT4,对肿瘤诊断、判断患者预后具有重要临床价值。
Objective: To investigate the expression of vascular endothelial growth factor C (VEGFC) and its receptor VEGFR3 (FLT4) in laryngeal and hypopharyngeal squamous cell carcinoma and their roles in lymphatic metastasis and micrometastasis. Methods: The expression of VEGFC protein and relative content of VEGFC, FLT4 and lymph nodes in 49 cases of laryngeal and hypopharyngeal carcinoma tissues were detected by immunohistochemical SP method and image analysis technique. Results: The expression of VEGFC in the laryngeal and hypopharyngeal tissues was closely related to the pathological grade (all P <0.05), and there was a correlation between the expression of VEGFC and the lymph node metastasis (all P <0.05), not related to the clinical T stage. The expression of FLT4 was positively correlated with the expression of VEGFC, which was correlated with the lymph node metastasis (all P <0.05). There was no significant difference in the number of FLT4 positive vessels and pathological grade and clinical T stage between laryngeal and hypopharyngeal tissues All P> 0.05). There was no significant difference between lymph node micrometastasis group, VEGFC, FLT4 expression and non-metastasis group in laryngeal and hypopharyngeal carcinoma tissues (P> 0.05). Conclusion: VEGFC plays an important role in tumor diagnosis and judging prognosis of patients by detecting the expression of VEGFC and FLT4 in the tissues of larynx and hypopharynx through FLT4, a specific receptor for FLT4, which leads to lymphangiogenesis and lymph node metastasis. Clinical Value.