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目的 :了解南通地区慢性丙型肝炎患者的HCV基因型分布情况。方法 :将 5 3例抗HCV阳性的血清标本用RT -PCR法检测HCV -RNA ,然后采用膜显色结果判读的基因芯片对 3 2例HCV -RNA阳性者进行基因分型研究。结果 :南通地区HCV感染检出 1b、1a、2a、1b + 1a、1b + 1a + 2a共 5种单一基因型和混合基因型 ,其中以 1b型为主 ,占 5 3 .1%。结论 :南通地区 1b型为优势株 ,2a和 1b + 1a型居次 ;1b + 1a型高于全国其他地区。
Objective: To understand the distribution of HCV genotypes in patients with chronic hepatitis C in Nantong area. Methods: HCV-RNA was detected by RT-PCR in 53 anti-HCV positive serum samples. Genotyping was performed on 32 HCV-RNA positive patients by gene chip analysis by colorimetric results. Results: A total of 5 single genotypes and mixed genotypes 1b, 1a, 2a, 1b + 1a, 1b + 1a + 2a were detected in HCV infection in Nantong area, of which 1b was the main type, accounting for 53.1%. Conclusion: Type 1b is the predominant strain in Nantong area, followed by type 2a and 1b + 1a, while type 1b + 1a is higher than the rest of the country.