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目的分析IRM-2小鼠全长cDNA文库的cDNA序列。方法根据IRM-2小鼠的21条EST,从IRM-2小鼠全长cDNA文库测定基于该21条EST序列PCR扩增的全长cDNA序列,通过与GenBank基因库同源序列信息比对,分析全长cDNA序列和性质。结果根据21对引物PCR产物的拼接,获得5条全长cDNA序列,与小鼠已知基因不同源,而且得到了可能编码蛋白质的氨基酸序列和蛋白质的性质。结论 5条全长cDNA序列提示IRM-2小鼠体内可能存在辐射抗性相关基因,为进一步分离和鉴定辐射抗性相关基因奠定了基础。
Objective To analyze the cDNA sequence of IRM-2 mouse full-length cDNA library. Methods Based on the 21 ESTs in IRM-2 mice, the full-length cDNA sequence amplified by PCR was obtained from the full-length cDNA library of IRM-2 mice. The full-length cDNA sequence was amplified by PCR from GenBank, Full-length cDNA sequences and properties were analyzed. Results According to the splicing of 21 pairs of PCR products, five full-length cDNA sequences were obtained, which were not homologous to the known genes in mice. The amino acid sequences and protein properties of the proteins were also obtained. Conclusion The five full-length cDNA sequences suggest that there may exist radiation-related genes in IRM-2 mice, which laid the foundation for further isolation and identification of radiation-related genes.