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用聚丙烯酰胺凝胶电泳分析表明,长春花植株感染泡桐丛枝病原(MLO)后茎叶中过氧化物同功酶总谱带数呈减少的趋势,健株最多产生11条酶带,通常具有6条典型的、稳定的谱带,即Ⅰ_b、Ⅰ_c、Ⅰ_d、Ⅱ、Ⅲ_d和Ⅲ_e;而表现典型的Ⅲ级症状的病株缺少低Rf值区的Ⅲ_d和Ⅲ_e带。Ⅲ_d和Ⅲ_e酶带的有无、浓度及活性与病株外部症状的严重度成负相关。七叶期前的健株幼苗茎叶中也无Ⅲ_d和Ⅲ_e谱带;随着植株生理年龄的增长,其活性和浓度逐渐增大。土霉素处理病株可以提高Ⅲ_d和Ⅲ_e酶带的活性和浓度。
Polyacrylamide gel electrophoresis analysis showed that the peroxidase isozyme bands in the leaves of P. vinifera plants tended to decrease after infected with the MLO, and the healthy plants had a maximum of 11 bands, There are six typical and stable bands, namely Ⅰ_b, Ⅰ_c, Ⅰ_d, Ⅱ, Ⅲ_d and Ⅲ_e; while the typical symptoms of the third-class symptoms of the lack of low Rf values Ⅲ_d and Ⅲ_e zone. Ⅲ_d and Ⅲ_e with or without enzyme band, concentration and activity of the external symptoms of the severity of the disease is negatively correlated. There was also no Ⅲ_d and Ⅲ_e bands in the stem and leaf of healthy plant before seedlings. With the increase of plant physiological age, its activity and concentration increased gradually. Oxytetracycline treatment of diseased plants can increase the Ⅲ_d and Ⅲ_e enzyme activity and concentration.