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Ｔｈ１和Ｔｈ２所分泌的具有代表性主要细胞因子分别为γ－ＩＦＮ和ＩＬ－４。在目前国内尚无条件测定Ｔｈ１／Ｔｈ２细胞表面标记的情况下，检测ｒ－ＩＦＮ和ＩＬ－４可大体了解Ｔｈ１／Ｔｈ２功能。本研究应用ＥＬＩＳＡ方法，采用晶美公司试剂，对不同临床类型病毒性肝炎患者清中γ－ＩＦＮ、ＩＬ－４及其比值进行检测和分析。结果发现：急性肝炎患者中，血清γ－ＩＦＮ和ＩＬ－４均明显提高，但前者提高的幅大于后者，故γ－ＩＦＮ／ＩＬ－４比值也提高。与之相比较，慢性肝炎患者血清中γ－ＩＦＮ和ＩＬ－４均明显减少，但因前者减少的程度大于后者，故γ－ＩＦＮ／ＩＬ－４比值明显降低。在重症肝炎患者中，血清γ－ＩＦＮ显著下降而血清ＩＬ－４显著增高，致使γ－ＩＦＮ／ＩＬ－４比值严重倒置。三型中γＩＦＮ的浓度从高到低依次为：急性、慢性、重症。ＩＬ－４的浓度依次为：急性、重症、慢性。γ－ＩＦＮ／ＩＬ－４比值的高低依次为：急性、慢性、重症ｈ１通过释放γ－ＩＦＮ而介导细胞免疫，Ｔｈ２则经ＩＬ－４介导体液免疫。Ｔｈ１／Ｔｈ２比值的平衡是维持正常免疫功能的重要因素。本研究结果表明Ｔｈ１／Ｔｈ２与病毒性肝炎疾病的转归有密切的关系。Ｔｈ１功能的增强有助于疾病好转，?
The major cytokines secreted by Th1 and Th2 are γ-IFN and IL-4, respectively. In the present situation of unconditioned determination of Th1 / Th2 cell surface markers, detection of r-IFN and IL-4 provides a general understanding of Th1 / Th2 function. In this study, the ELISA method was used to detect and analyze the serum levels of γ-IFN, IL-4 and their ratios in patients with different clinical types of viral hepatitis using Jingmei Reagents. The results showed that the serum levels of γ-IFN and IL-4 were significantly increased in patients with acute hepatitis, but the former increased more than the latter, and the ratio of γ-IFN / IL-4 also increased. In contrast, serum levels of γ-IFN and IL-4 were significantly decreased in patients with chronic hepatitis, but the ratio of γ-IFN / IL-4 was significantly lower because the former was more decreased than the latter. In patients with severe hepatitis, serum γ-IFN was significantly decreased serum IL-4 significantly increased, resulting in a serious inversion of γ-IFN / IL-4 ratio. Three types of γ IFN concentration in descending order were: acute, chronic, severe. IL-4 concentrations were as follows: acute, severe, chronic. The ratios of γ-IFN / IL-4 were as follows: acute, chronic and severe h1 mediated cellular immunity by releasing γ-IFN, while Th2 was mediated by IL-4 humoral immunity. The balance of the Th1 / Th2 ratio is an important factor in maintaining normal immune function. The results of this study show that Th1 / Th2 is closely related to the outcome of viral hepatitis. Th1 function enhancements help to improve the disease?