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目的研究苯并(a)芘[Benzo(a)pyrene,B(a)P]暴露对职业人群尿中1-羟基芘(1-OH-Py)及外周血CYP1A1mRNA水平的影响,探讨两者之间的关系。方法选取118名焦化厂车间作业工人接触组和63名非职业接触人群为对照组进行问卷调查;收集研究对象工作班后6h尿样,晨起空腹抽取静脉血。用高效液相色谱法-荧光检测器法检测尿中1-OH-Py的含量,QT-PCR检测外周血细胞中CYP1A1 mRNA水平。结果接触组1-OH-Py为(0.623±0.762)μmol/mol Cr,对照组为(0.272±0.231)μmol/mol Cr,差异有统计学意义(P<0.05)。接触组CYP1A1水平为0.309±0.552,对照组为0.479±0.586,差异有统计学意义(P<0.05)。尿1-OH-Py水平与外周血CYP1A1的基因表达水平之间呈负相关(r=-0.219,P=0.017)。结论尿中1-OH-Py的水平可以作为职业暴露人群的外暴露生物标志物,接触B(a)P的职业人群外周血CYP1A1 mRNA表达水平降低,且两者之间有相关性。
Objective To investigate the effects of benzo (a) pyrene [Benzo (a) pyrene, B (a) P] exposure on urinary 1-OH-Py and CYP1A1 mRNA levels in occupational populations, Relationship between. Methods A total of 118 workers in the workshop of coking plant and 63 non-occupational exposure groups were selected as the control group to conduct questionnaire survey. Urine samples were collected 6 hours after the working class of study and fasting venous blood samples were collected in the morning. The content of 1-OH-Py in urine was detected by HPLC-fluorescence detector, and the level of CYP1A1 mRNA in peripheral blood was detected by QT-PCR. Results The 1-OH-Py group was (0.623 ± 0.762) μmol / mol Cr in the exposure group and 0.272 ± 0.231 μmol / mol Cr in the control group (P <0.05). The contact group CYP1A1 level was 0.309 ± 0.552, the control group was 0.479 ± 0.586, the difference was statistically significant (P <0.05). There was a negative correlation between urinary 1-OH-Py levels and CYP1A1 gene expression in peripheral blood (r = -0.219, P = 0.017). Conclusions The level of urinary 1-OH-Py may be used as a biomarker of exposure to occupational exposure. CYP1A1 mRNA expression is decreased in the occupational population exposed to B (a) P, and there is a correlation between the two.