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目的:研究MK-447对家兔凝血酶诱导的血小板聚集释放反应及单细胞内钙水平的影响.方法:利用浊度法及测定PRP中ATP的含量评价聚集和释放反应,以荧光图像法分析细胞内钙浓度.结果:MK-447仅使兔多血小板血浆(PRP)透光度降低(DLT),即血小板变形,单血小板[Ca~(2+)]_i轻度增加(160 nmol·L~(-1)),并不被依他酸3 mmol·L~(-1)抑制.MK-447消除凝血酶诱导的DLT,聚集和ATP释放增强,呈剂量依赖性,且凝血酶介导的[Ca2+]_i由369 ±45 nmol·L~(-1)增加到621±121 nmol·L~(-1).结论:MK-447的血小板变形与其[Ca~(2+)]_i释放有关.MK-447增强凝血酶的血小板聚集和ATP释放.MK-447的这一作用可能于[Ca2+]_i的协同作用有关.
Objective: To study the effect of MK-447 on thrombin-induced platelet aggregation-release reaction and single-cell intracellular calcium level in rabbit.Methods: The aggregation and release responses were evaluated by turbidimetric method and the determination of ATP content in PRP. Fluorescent image analysis Intracellular calcium concentration.Results MK-447 decreased the transmissivity of platelet-rich plasma (PRP) in rabbits only, that is, the platelet deformity and the slight increase of platelet [Ca 2+] i (160 nmol·L ~ (-1)) was not inhibited by 3 mmol·L -1 ethacryate.MK-447 enhanced thrombin-induced DLT, aggregation and ATP release in a dose-dependent manner, and thrombin-mediated Of [Ca2 +] i increased from 369 ± 45 nmol·L -1 to 621 ± 121 nmol·L -1 Conclusions: The platelet degeneration of MK-447 is not related to the release of [Ca 2+] i MK-447 enhances platelet aggregation and ATP release from thrombin, and this effect of MK-447 may be related to the synergistic effect of [Ca2 +] i.