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目的 探讨心肌细胞坏死在心脏移植中的发生和发展情况， 为心脏移植的抗排斥反应治疗和预防提供依据。方法 用供体脾细胞（ＳＰＣ） 和环磷酰胺（ＣＰ） 预处理移植受体， ＨＥ染色对移植心脏的炎细胞浸润和心肌细胞坏死进行分析。结果 经ＳＰＣ和ＣＰ预处理后， 移植心脏的存活时间明显延长， 炎细胞浸润和心肌细胞变性坏死明显减轻。结论 （１） 急性排斥反应时， 移植心脏内大量的炎细胞浸润和心肌细胞坏死。（２） 大鼠心脏移植的急性排斥反应是由心外膜周围起始逐渐向心内膜方向发展。（３） ＳＰＣ和ＣＰ联合预处理， 可以预防和减缓排斥反应。
Objective To investigate the occurrence and development of cardiomyocyte necrosis in heart transplantation and to provide basis for anti-rejection therapy and prevention of heart transplantation. Methods The donor spleen cells (SPC) and cyclophosphamide (CP) were used to pretreat the transplanted recipients, and the HE staining was used to analyze the inflammatory cell infiltration and cardiomyocyte necrosis in the transplanted heart. Results After pretreatment with SPC and CP, the survival time of transplanted heart was significantly prolonged, inflammatory cell infiltration and cardiomyocyte degeneration and necrosis were significantly reduced. Conclusions (1) During acute rejection, a large number of infiltration of inflammatory cells and necrosis of cardiomyocytes were transplanted in the heart. (2) The acute rejection in rat heart transplantation is from the beginning of epicardial to the endocardial. (3) SPC and CP combined pretreatment can prevent and slow the rejection.