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目的：证实鬼臼毒素自旋标记物ＧＰ４和ＧＰ７体外对Ｍｏｌｔ４Ｂ细胞周期和大分子合成的抑制作用．方法：ＭＴＴ、同位素标记法和流式细胞分光光度仪ＢｒｄＵ／ＤＮＡ分析法．结果：ＧＰ４，ＧＰ７和依托泊苷（００２－１００ｍｍｏｌ·Ｌ－１，４８ｈ）抑制细胞生长量效曲线不同，其ＩＣ５０值分别为０．１１，４．７，１．６ｍｍｏｌ·Ｌ－１，并降低ＤＮＡ、蛋白质合成．ＧＰ４（１０ｍｍｏｌ·Ｌ－１１２ｈ）增加有丝分裂指数，为对照组的５倍；ＧＰ７和依托泊苷都降低有丝分裂指数，分别为对照组的１／３和１／１０．ＧＰ４主要阻止细胞于Ｇ２／Ｍ期，而ＧＰ７与依托泊苷相似，将细胞阻滞在Ｓ和Ｇ２期．结论：ＧＰ４和ＧＰ７抑制Ｍｏｌｔ４Ｂ细胞生长和大分子合成，其作用机制不同
OBJECTIVE: To demonstrate the inhibitory effect of podophyllotoxin spin markers GP4 and GP7 on Molt4B cell cycle and macromolecular synthesis. Methods: MTT, isotope labeling, and flow cytometric spectrophotometry BrdU/DNA analysis. Results: The dose-response curves of GP4, GP7 and etoposide (002-100 mmol·L-1, 48h) inhibited cell growth were different, and their IC50 values were 0.11, 4.7, 1.6 mmol·L-1, respectively. , And reduce DNA, protein synthesis. GP4 (10 mmol·L-112h) increased the mitotic index five times that of the control group; both GP7 and etoposide decreased the mitotic index, which was 1/3 and 1/10 of the control group, respectively. GP4 mainly prevented cells in the G2/M phase, whereas GP7 was similar to etoposide, arresting cells in the S and G2 phases. CONCLUSION: GP4 and GP7 inhibit the growth and macromolecular synthesis of Molt4B cells, and their mechanisms of action are different.