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背景:羊膜细胞主要由羊膜上皮细胞和羊膜间充质细胞组成,均具有多分化潜能,可转化为神经元,且还有合成、释放生物活性物质和神经营养因子的功能。作者前期研究证实羊膜细胞移植入脑内后,能明显促进脑内神经元的再生。目的:探索羊膜细胞对小鼠缺血再灌注损伤脑细胞的作用。方法:将Balb/C小鼠通过夹闭双侧颈总动脉方法建立脑缺血再灌注损伤模型后,分离小鼠脑细胞。取孕鼠新鲜胎盘,分离羊膜细胞。将与羊膜细胞共培养的小鼠脑细胞作为实验组,以PBS培养的小鼠脑细胞作为对照组。结果与结论:实验组小鼠脑细胞活性较对照组明显增加(P<0.05)。培养24,72 h后实验组小鼠脑细胞坏死率较对照组差异无显著性意义(P>0.05),而培养48 h后实验组小鼠脑细胞坏死率较对照组明显降低(P<0.05)。实验组小鼠脑细胞中S期细胞数量增加,而对照组小鼠脑细胞中G1期细胞数量增加,S期细胞数量减少,但2组小鼠脑细胞中G2期细胞数量不变。说明羊膜细胞具有保护缺血再灌注损伤Balb/C小鼠脑细胞的作用,且能抑制其坏死和凋亡并促进其再生。
BACKGROUND: Amniotic cells are mainly composed of amniotic epithelial cells and amniotic mesenchymal cells, all of which have pluripotency potential and can be transformed into neurons. They also have functions of synthesizing and releasing bioactive substances and neurotrophic factors. Previous studies confirmed that amniotic cells transplanted into the brain, can significantly promote the regeneration of neurons in the brain. Objective: To explore the effect of amniotic cells on cerebral ischemia-reperfusion injury in mice. METHODS: Balb / C mice were implanted with bilateral common carotid arteries to establish cerebral ischemia-reperfusion injury model and mouse brain cells were isolated. Pregnant mice take fresh placenta, amniotic cells isolated. Mouse brain cells co-cultured with amniotic cells were used as experimental group and mouse brain cells cultured in PBS as control group. RESULTS AND CONCLUSION: The activity of brain cells in experimental group was significantly higher than that in control group (P <0.05). Compared with the control group, the necrosis rate of brain cells in experiment group was not significantly different (P> 0.05) at 24 and 72 hours after culturing, but the necrosis rate of brain cells in experimental group was significantly lower than that of control group ). In experimental group, the number of S phase cells in brain cells increased, while in control group, the number of G1 phase cells increased and the number of S phase cells decreased in control group, but the number of G2 phase cells in the two groups remained unchanged. These results indicate that amniotic cells can protect the brain cells of Balb / C mice from ischemia-reperfusion injury and can inhibit their necrosis and apoptosis and promote their regeneration.