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目的切除山羊双侧卵巢(OVX山羊)建立绝经后骨质疏松(PMO)动物模型,应用骨组织形态计量学方法观察OVX山羊对四环素-雌酮(XW630)的治疗反应。方法31只雌性山羊随机分成4组,即正常对照组、假手术组、OVX术后6个月组、XW630治疗6个月组,制备山羊髂骨不脱钙骨切片,应用骨组织形态计量学方法,观察各组髂骨骨计量学参数的变化。结果正常对照组和假手术组比较各参数间差异无显著性。与正常对照组和假手术组比较,OVX术后6个月组的骨小梁体积比全部骨组织体积(TBV/TTV)、骨小梁体积比海绵骨体积(TBV/SBV)、平均骨小梁板厚度(MTPT)、四环素双标线距离(DDL)、平均类骨质宽度(MOSW)、骨矿化沉积率(MiAR)和组织水平的骨形成速率(Svf)显著减少(Svf:P<0.05,其余各参数:P<0.01),骨小梁表面比体积(S/V)则显著增加(P<0.01),说明去卵巢后山羊骨量丢失,骨小梁体积明显减小,骨小梁厚度下降、连接性降低,骨转换率降低,激活频率减慢,骨形成明显减少,表现为低转化型骨质疏松的骨代谢特征。骨质疏松山羊模型复制成功。与OVX术后6个月组相比,XW630治疗6个月组的TBV/TTV,TBV/SBV,MTPT,DDL,MOSW,MiAR和Svf显著增加(P<0.01),S/V,骨矿化延迟时间(MLT)显著减少(P<0.01);与假手术组相比,XW630治疗6个月组的TBV/TTV,TBV/SBV显著减少(P<0.01)。结论XW630有增加骨量,改善骨小梁空间结构,增加骨强度,提高骨激活频率,促进类骨质生成及矿化,增强成骨细胞活性的作用。
OBJECTIVE: To establish an animal model of postmenopausal osteoporosis (PMO) by excising bilateral goat ovaries (OVX goats) and observe the therapeutic response of tetracycline-estrone (XW630) to OVX goats using bone histomorphometry. Methods Thirty-one female goats were randomly divided into 4 groups: normal control group, sham operation group, 6-month OVX group and 6-month XW630 group. Goat iliac non-decalcified bone slices were prepared and bone histomorphometry Methods The changes of the ilium bone metrology parameters in each group were observed. Results There was no significant difference between the normal control group and sham operation group in each parameter. Compared with the normal control group and the sham operation group, the trabecular volume of the 6-month OVX group was significantly lower than that of the TBV / TTV group, TBV / SBV group, MTPT, DDL, MOSW, MiAR, and Svf decreased significantly (Svf: P < 0.05, the rest parameters: P <0.01), and the trabecular surface specific volume (S / V) increased significantly (P <0.01), which indicated that the bone mass of ovariectomized sheep was decreased, the trabecular bone volume was significantly reduced, Decreased beam thickness, decreased connectivity, reduced bone turnover rate, slower activation frequency, and markedly reduced bone formation are characteristic of bone metabolism in low-turnover osteoporosis. Osteoporosis goat model copied successfully. TBV / TTV, TBV / SBV, MTPT, DDL, MOSW, MiAR and Svf were significantly increased (P <0.01), S / V and bone mineralization were significantly increased in 6-month XW630- (P <0.01). Compared with the sham operation group, the TBV / TTV and TBV / SBV of the 6-month XW630 treatment group decreased significantly (P <0.01). Conclusion XW630 can increase the bone mass, improve the spatial structure of trabecular bone, increase the bone strength, increase the frequency of bone activation, promote osteoid formation and mineralization and enhance the activity of osteoblasts.