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目的:探讨在鸡胚脊髓发育早期背侧抑制性轴突导向蛋白(draxin)对脊髓背侧中间神经元(dorsal in-terneurons,dI)迁移特性的影响。方法:应用免疫组织化学的方法观察鸡胚脊髓dI的发育特性;应用电穿孔的方法在鸡胚一侧脊髓内过表达draxin,观察draxin过表达后对鸡胚脊髓内dI神经元发育特性的影响。结果:随着胚胎的发育,鸡胚脊髓内dI3中间神经元首先在脊髓背侧区形成并逐渐向腹侧迁移,而dI2、dI4和dI6中间神经元没有形成明显的腹侧迁移特性;鸡胚脊髓内分别过表达分泌型和跨膜型draxin时,dI3中间神经元的腹侧迁移延迟,且在跨膜型draxin过表达时其受影响程度较高;而dI2、dI4和dI6中间神经元的迁移未受到明显影响。结论:Draxin参与鸡胚脊髓内dI3中间神经元腹侧迁移的调节。
Objective: To investigate the effect of dorsal inhibitory axin (draxin) on the migratory characteristics of dorsal in-terneurons (dI) in the early development of chicken embryo. Methods: The developmental characteristics of dI in chicken embryo spinal cord were observed by immunohistochemistry. The expression of draxin was overexpressed in the spinal cord on the side of chicken embryo by electroporation. The effect of draxin overexpression on the development of dI neurons in chicken embryo spinal cord . Results: With the development of embryos, dI3 interneurons in chicken embryo spinal cord first formed in the dorsal spinal cord area and gradually migrated to the ventral, while dI2, dI4 and dI6 interneurons did not develop significant ventral migratory characteristics; chicken embryos In the spinal cord, the secretory and transmembrane drains were overexpressed, ventral migration of dI3 interneurons was delayed and was more affected by transmembrane draxin overexpression. However, dI2, dI4 and dI6 interneurons Migration has not been significantly affected. Conclusion: Draxin is involved in the ventral migration of dI3 interneurons in chicken spinal cord.