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用结晶紫染色法测定了肿瘤坏死因子－β（ＴＮＦ－β）单独应用及与细胞因子γ－干扰素（ＩＦＮ－γ）、白细胞介素－２（ＩＬ－２）和与抗肿瘤药物丝裂霉素（ＭＭＣ）、阿霉素（ＤＸＲ）、顺铂（ＣＤＤＰ）联合应用，对体外培养的人肺腺癌细胞株ＡＧＺＹ－８３ａ细胞的抗增殖作用。结果表明，ＴＮＦ－β有抗肺癌细胞增殖的作用，其强度为（３６．６±４．３）％；与细胞因子或抗肿瘤药物伍用能增强其作用，在抗肿瘤药物中以与阿霉素配伍为最佳，抗增殖作用可达到（６６．１±８．４）％。
The use of tumor necrosis factor-β (TNF-β) alone and in combination with cytokines interferon-γ (IFN-γ), interleukin-2 (IL-2) and antitumor drugs was determined by crystal violet staining The anti-proliferative effects of MMC, DXR, and CDDP on human lung adenocarcinoma cell line AGZY-83a in vitro. The results showed that TNF-β had anti-proliferative effects on lung cancer cells with an intensity of (36.6±4.3)%. The use of TNF-β in combination with cytokines or antitumor drugs can enhance its effect, and it can be used as an antitumor drug. The optimal combination of antimyelosin was anti-proliferative effect (66.1±8.4)%.