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从ＰＯＪ３０１６、Ｆ１３４、桂糖１１号、ＲＯＣ１０与云南割手密种、斑茅种、滇蔗茅种远缘杂交的大批杂交后代中，按组合关系选取９个材料进行过氧化物同工酶亲子关系真实性鉴定研究，并进行酶带分析比较。结果显示：①云割斑Ｆ２９５／６９等４个材料属于ＲＯＣ１０近亲杂交亲子关系的可能性很大；②云滇Ｆ１９１／２００８、云割斑滇Ｆ１９９／４７等５个材料属于云南野生甘蔗资源属种间远缘杂交亲子关系．同工酶技术用于血缘关系复杂的亲子关系真实性鉴定有简便、清晰、可靠的优点，但要提高正确判断的水平，还必须有丰富的育种经验，将亲子代特征特性变异的识别与大量的酶带分析实践相结合。涉及理论研究的材料必须进行分子技术鉴定．
Nine progeny from the cross combinations of POJ3016, F134, Guitang 11, ROC10 and Yunnan cut mongolia, Dahong Mao and Dianmu Mao were selected for 9 combinations of peroxidase isozymes Relationship authenticity identification studies, and analysis of the enzyme band. The results showed that: (1) There were a high probability that four materials such as F295 / 69 were part of the close relatives of ROC10. (2) Five materials, F191 / 2008 and F199 / 47, Interspecific hybrid parent-child relationship. Isoenzyme technology for the relationship between the complex kinship authenticity identification has the advantages of simple, clear and reliable, but to improve the correct level of judgment, but also must have rich experience in breeding, the parent-offspring of the characteristics of variation identification and a large number of The combination of enzyme band analysis practice. Materials involved in theoretical studies must be molecular identification.