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为增强肝癌肿瘤浸润淋巴细胞（ＴＩＬ）的趋肝性，制备了半乳糖基抗ＣＤ３单克隆抗体－ＴＩＬ（Ｇａｌ－ａｎｔｉＣＤ３ＭｃＡｂ－ＴＩＬ），将其与肝细胞共同孵育，在倒置显微镜下观察两者通过无唾液酸糖蛋白受体介导的作用情况。测定了半乳糖基抗ＣＤ３单抗（Ｇａｌ－ａｎｔｉＣＤ３ＭｃＡｂ）的糖密度以及Ｇａｌ－ａｎｔｉＣＤ３ＭｃＡｂ与ＴＩＬ的结合率。结果发现，Ｇａｌ－ａｎｔｉＣＤ３ＭｃＡｂ－ＴＩＬ与肝细胞有明显粘附现象。Ｇａｌ－ａｎｔｉＣＤ３ＭｃＡｂ的糖密度为６２．１８，Ｇａｌ－ａｎｔｉＣＤ３ＭｃＡｂ与ＴＩＬ的结合率为９７．９％。结果表明，Ｇａｌ－ａｎｔｉＣＤ３ＭｃＡｂ－ＴＩＬ在体外有良好的趋肝性，６２．１８的糖密度和９７．９％的结合率保证了ＴＩＬ的有效利用。
In order to enhance the hepatotropism of hepatoma tumor infiltrating lymphocytes (TIL), a galactosyl anti-CD3 monoclonal antibody-TIL (Gal-antiCD3 McAb-TIL) was prepared, incubated with hepatocytes, and observed under an inverted microscope. Through the asialoglycoprotein receptor-mediated effect. The sugar density of galactosyl anti-CD3 monoclonal antibody (Gal-antiCD3 McAb) and the binding rate of Gal-antiCD3 McAb to TIL were measured. As a result, it was found that Gal-antiCD3 McAb-TIL had a significant adhesion to hepatocytes. The sugar density of Gal-antiCD3 McAb was 62.18, and the binding rate of Gal-antiCD3 McAb to TIL was 97.9%. The results showed that Gal-antiCD3McAb-TIL had good hepatotropism in vitro, and the sugar density of 62.18 and the binding rate of 97.9% ensured the effective use of TIL.