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1984年1月10日从上海动物园的猩猩(Ponge pygmaeus)粪便中检获一种鞭毛虫,即取其新鲜粪便接种于洛克液琼脂血清培养基中,共接种两管,每一培养管各加入青霉素2500单位,链霉素2.5mg;培养于35℃±0.5℃,三天后第一次移种时,培养管不加抗菌素,此后每周转种二次,至10月份连续培养80余代,该虫生长良好,说明这种鞭毛虫可适应于体外培养,所用的这种培养基也较合适。
On January 10, 1984, a flagellate was seized from the feces of the Ponge pygmaeus at Shanghai Zoo. The fresh faeces were inoculated on a Lockeed agar serum medium and inoculated with two tubes. Each tube was filled with penicillin 2500 units, streptomycin 2.5mg; cultured at 35 ℃ ± 0.5 ℃, three days after the first transplanted, the culture tube without antibiotics, then transplanted twice a week until October for more than 80 generations of culture, the insect Good growth, indicating that the flagellates can adapt to in vitro culture, the medium used is more appropriate.