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AIM:To explore the etiologic role of HPV infection inesophageal carcinoma,and the association of HPV-16 E6with the nuclear matrix of carcinoma cells.METHODS:Two esophageal carcinoma cell lines,EC/CUHK1 and EC/CUHK2,were tested for HPV-16 E6subgenetic fragment by polymerase chain reactionarnplification of virus DNA associated nuclear matrix.RT-PCR and immunocytochemistry were also used to visualizethe expression of E6 subgene in the cells.RESULTS:The HPV-16 E6 subgenetic fragment was found tobe present in nuclear matrix-associated DNA,E6oncowprotein localized in the nucleus where it is tightlyassociated with nuclear matrix after sequential extraction inEC/CUHK2 cells.It was not detected,however,in EC/CUHK1 cells.CONCLUSION:The interaction between HPV-16 E6 andnuclear matrix may contribute to the virus inducedcarcinogenesis in esophageal carcinoma.
AIM:To explore the etiologic role of HPV infection inesophageal carcinoma,and the association of HPV-16 E6 with the nuclear matrix of carcinoma cells.METHODS:Two esophageal carcinoma cell lines,EC/CUHK1 and EC/CUHK2,were tested for HPV-16 E6subgenetic fragment by polymerase chain reactionarnplification of virus DNA associated nuclear matrix.RT-PCR and immunocytochemistry were also used to visualizethe expression of E6 subgene in the cells.RESULTS:The HPV-16 E6 subgenetic fragment was found tobe present in nuclear matrix-associated DNA ,E6oncowprotein localized in the nucleus where it is tightlyassociated with nuclear matrix after sequential extraction inEC/CUHK2 cells.It was not detected,however,in EC/CUHK1 cells.CONCLUSION:The interaction between HPV-16 E6 andnuclear matrix may contribute to the virus Inducedcarcinogenesis in esophageal carcinoma.