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目的:观察氟对大鼠牙及骨代谢的影响,探讨氟斑牙的发病机制。方法:48只Wistar大鼠随机分为4组,每组12只。染氟组分别饮用含氟化钠浓度为50、100、150 mg/L的自来水,对照组饮用常规自来水。8周后麻醉处死,观察大鼠氟斑牙的发病情况。应用放射免疫法测定血清骨钙素(OC)、甲状旁腺素(PTH)、降钙素(CT)水平:氟离子选择电极法测定血氟及牙氟浓度,生化方法测定大鼠血清钙含量。采用SPSS13.0软件包对氟斑牙检出率进行x~2检验,单因素方差分析比较各指标组间差异。结果:各染氟组大鼠血氟、牙氟含量显著高于对照组(P<0.05),差异具有显著性,而且各组氟含量随饮水氟浓度的增高而逐渐增高(F值分别为11.234、275.148,P<0.01)。染氟组大鼠血清钙含量显著低于对照组(P<0.05),且各组血钙含量随饮水氟浓度的增高而逐渐降低(F=3.906,P<0.05)。中、高剂量组大鼠血清PTH、OC含量显著高于对照组,差异具有显著性(P<0.01);各组含量随饮水氟浓度的增高而逐渐增高,组间差异显著(F值分别为8.548、3.801,P<0.05)。低、中剂量组大鼠血清CT呈下降趋势,但与对照组相比并无显著差异(P>0.05);而高氟剂量组CT比对照组显著下降(P<0.01)。各组血清CT含量的组间差异显著(F=5.121,P<0.05)。结论:氟影响大鼠的牙及骨代谢.OC、PTH及CT在氟牙症发病中起着重要作用。
Objective: To observe the effects of fluoride on rat teeth and bone metabolism and to explore the pathogenesis of dental fluorosis. Methods: Forty-eight Wistar rats were randomly divided into 4 groups with 12 rats in each group. Dye fluoride group were drinking tap water containing sodium fluoride concentration of 50,100,150 mg / L, the control group to drink regular tap water. After 8 weeks, the rats were sacrificed to observe the incidence of dental fluorosis in rats. Serum levels of osteocalcin (OC), parathyroid hormone (PTH) and calcitonin (CT) were measured by radioimmunoassay: blood fluoride and fluoride concentration were measured by fluoride ion selective electrode method, serum calcium concentration . The detection rate of dental fluorosis by SPSS 13.0 software package was tested by x ~ 2, and the variance of each index was compared by one-way ANOVA. Results: The levels of fluoride and fluoride in blood of each group were significantly higher than those of the control group (P <0.05), and the difference was significant. The fluoride content in each group increased gradually with the increase of fluoride concentration in drinking water (F = 11.234 , 275.148, P <0.01). The content of serum calcium in fluorosis group was significantly lower than that in control group (P <0.05), and the content of serum calcium in each group decreased gradually with the increase of fluoride concentration in drinking water (F = 3.906, P <0.05). The levels of serum PTH and OC in the middle and high dose groups were significantly higher than those in the control group (P <0.01). The content of each group increased gradually with the increase of fluoride concentration in drinking water 8.548, 3.801, P <0.05). Serum CT of low and middle dose groups showed a decreasing trend, but there was no significant difference compared with the control group (P> 0.05), while CT in high fluoride group was significantly lower than that of the control group (P <0.01). The serum CT contents in each group differed significantly (F = 5.121, P <0.05). Conclusion: Fluoride affects dental and bone metabolism in rats, and OC, PTH and CT play an important role in the pathogenesis of dental fluorosis.