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目的探讨消岩汤治疗肺癌恶病质的可能作用机制。方法将40只小鼠随机分为健康组、模型组、消岩汤组、醋酸甲地孕酮组,每组10只。除健康组外,其余各组通过接种肿瘤细胞建立肺癌恶病质模型。造模成功后模型组小鼠每日灌服生理盐水1 ml,消岩汤组每日灌服消岩汤0.42 g,醋酸甲地孕酮组每日灌服醋酸甲地孕酮3.75 mg/d,均给药14天。测定腓肠肌重量,血清肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)含量及肌肉萎缩盒F基因(MAFbx)和肌肉环状指基因1(MURF-1)m RNA表达。结果与健康组比较,模型组腓肠肌重量下降,模型组和醋酸甲地孕酮组TNF-α、IL-6水平及MAFbx、MURF-1m RNA表达均明显上升(P<0.01);与模型组比较,消岩汤组腓肠肌重量明显增加,血清TNF-α、IL-6水平及MAFbx、MURF-1 m RNA表达均明显下降(P<0.05或P<0.01),醋酸甲地孕酮组TNF-α、IL-6水平均明显下降(P<0.05);醋酸甲地孕酮组腓肠肌重量明显低于消岩汤组(P<0.01),血清TNF-α、IL-6水平及MAFbx、MURF-1 m RNA表达明显高于消岩汤组(P<0.05或P<0.01)。结论消岩汤可能通过减少炎症细胞因子产生,抑制MAFbx和MURF-1基因表达,改善肺癌恶病质肌肉蛋白质降解,从而改善肺癌恶病质状态。
Objective To explore the possible mechanism of Xiao Yan Decoction in treating cachexia of lung cancer. Methods Forty mice were randomly divided into healthy group, model group, Huanan decoction group and megestrol acetate group, with 10 mice in each group. Except for the healthy group, all the other groups established the lung cancer cachexia model by inoculating tumor cells. After successful modeling, the mice in model group were fed with 1 ml of normal saline daily, while Qiaoyan Decoction group was given 0.42 g of diamazepam daily. The megestrol acetate group was fed with 3.75 mg / , Were given 14 days. The gastrocnemius muscle weight, serum tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) content and muscle FIBx gene expression and muscle ring finger gene 1 (MURF-1) m RNA expression were measured. Results Compared with healthy group, the weight of gastrocnemius muscle decreased in model group and the levels of TNF-α and IL-6 and the expression of MAFbx and MURF-1m RNA in model group and megestrol acetate group were significantly increased (P <0.01). Compared with model group (P <0.05 or P <0.01). The levels of TNF-α and IL-6 in serum and the expression of MAFbx and MURF-1 m RNA in the Xiaoyan decoction group were significantly increased (P <0.01). The levels of TNF-α, IL-6 and the levels of MAFbx, MURF-1 and IL-6 in the megestrol acetate group were significantly lower than those in the control group The m RNA expression was significantly higher than that of HuanYan decoction (P <0.05 or P <0.01). CONCLUSION Xiao-Yan-Tang may improve the cachexia status of lung cancer by reducing the production of inflammatory cytokines, inhibiting the expression of MAFbx and MURF-1 gene, improving the muscle protein degradation of cachexia in lung cancer.