A set of universal loop-mediated isothermal amplification(LAMP)primers targeting the fla gene was designed to detect Borrelia burgdorferi sensu lato(B.burgdorferi s.l.)in human samples.The sensitivity of LAMP was 20 copies/reaction,and the assay did not detect false positives among 11 other related bacteria.A positive LAMP result was obtained for 9 of the 24 confirmed cases and for 12of 94 suspected cases.The positive rate of LAMP The set of universal loop-mediated isothermal amplification (LAMP) primers targeting the fla gene was designed to detect Borrelia burgdorferi sensu lato (B. burgdorferi sl) in human samples. Sensitivity of LAMP was 20 copies / reaction, and the assay did not detect false positives among 11 other related bacteria. A positive LAMP result was obtained for 9 of the 24 confirmed cases and for 12 of 94 susceptive cases. the positive rate of LAMP