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目的:研究比较神经纤毛蛋白1(NRP-1)反义寡核苷酸(ASODN)与血管内皮生长因子受体2(VEGFR-2)反义寡核苷酸(ASODN)对人胃癌SGC7901细胞增殖活性及凋亡水平的影响。方法:分别及同时将不同浓度经硫代磷酸化修饰的NRP-1 ASODN和VEGFR-2 ASODN转染入人胃癌SGC7901细胞,逆转录-聚合酶链反应(RT-PCR)检测NRP-1基因和VEGFR-2基因mRNA的转录水平;MTT比色法测量细胞的增殖活性;流式细胞仪测量细胞的凋亡水平。结果:转染NRP-1ASODN和VEGFR-2 ASODN后,人胃癌SGC7901细胞NRP-1基因和VEGFR-2基因mRNA的转录水平均出现降低;NRP-1 ASODN和VEGFR-2 ASODN对SGC7901细胞有明显抑制增殖和促进凋亡的作用,且随着ASODN浓度升高而增强;分别转染时其作用无显著差别,联合转染时其作用明显增强。结论:NRP-1 ASODN和VEGFR-2 ASODN可抑制人胃癌SGC7901细胞NRP-1基因和VEGFR-2基因mRNA的转录水平及细胞增殖活性,促进细胞凋亡;与分别转染相比,两者联合转染作用明显增强。
OBJECTIVE: To study the effects of ASODN and VEGFR-2 antisense oligonucleotide (ASODN) on the proliferation of human gastric cancer cell line SGC7901 Activity and apoptosis levels. METHODS: Human gastric cancer cell line SGC7901 was transfected with different concentrations of NRP-1 ASODN and VEGFR-2 ASODN modified by thiophosphorylation respectively. The expression of NRP-1 gene and its mRNA were detected by reverse transcription-polymerase chain reaction VEGFR-2 gene mRNA transcription level; MTT colorimetric assay of cell proliferation activity; flow cytometry cell apoptosis levels. Results: The transcript levels of NRP-1 gene and VEGFR-2 gene in human gastric cancer cell line SGC7901 were decreased after NOD-1 ASODN and VEGFR-2 ASODN transfection. NRP-1 ASODN and VEGFR-2 ASODN significantly inhibited SGC7901 cells Proliferation and apoptosis, and increased with the increase of ASODN concentration. There was no significant difference between the two groups when transfected with ASODN. CONCLUSIONS: NOD-1 ASODN and VEGFR-2 ASODN can inhibit the mRNA and protein expression of NRP-1 and VEGFR-2 in human gastric cancer cell line SGC7901 and promote their apoptosis. Compared with transfection, NRP-1 ASODN and VEGFR- Transfection was significantly enhanced.