目的采用固相萃取结合液相色谱-质谱法检验人全血中地塞米松。方法采用SPE提取全血,用UPLC-MS/MS方法测定,采用ESI离子源,MRM方式监测。结果地塞米松的检出限为0.05ng/mL,线性范围1ng/mL~100ng/mL,方法回收率大于78.1%,日内、日间精密度均小于15%。结论 SPE-UPLC/MS/MS检验人全血中地塞米松的方法简单、高效,可应用于人全血中地塞米松的检验。 Objective To determine dexamethasone in human whole blood by solid-phase extraction combined with liquid chromatography-mass spectrometry. Methods Whole blood was extracted by SPE and determined by UPLC-MS / MS, and monitored by ESI source and MRM. Results The detection limit of dexamethasone was 0.05ng / mL and the linear range was 1ng / mL ~ 100ng / mL. The recovery rate of the method was more than 78.1%. The intra- and inter-day precision was less than 15%. Conclusion The SPE-UPLC / MS / MS method for the determination of dexamethasone in human whole blood is simple and effective and can be applied to the test of dexamethasone in human whole blood.