论文部分内容阅读
目的:通过力竭性游泳实验观察大鼠心肌房室束的组织化学改变,探讨力竭训练对心传导系统的影响。方法:力竭性游泳后取房室束分别进行糖原、乳酸脱氢酶、琥珀酸脱氢酶、单胺氧化酶、乙酰胆碱脂酶、碱性磷酸酶染色,并采用图像分析系统测定组化反应的OD值。结果:与对照组相比,一次力竭性游泳后心肌房室束糖原含量下降,乳酸脱氢酶活性增强,琥珀酸脱氢酶、单胺氧化酶、乙酰胆碱脂酶和碱性磷酸酶的活性均减弱;与一次力竭组相比,1周力竭游泳后心肌房室束糖原含量上升,乳酸脱氢酶、琥珀酸脱氢酶和单胺氧化酶活性均减弱,乙酰胆碱脂酶和碱性磷酸酶活性增强。结果提示一次力竭性游泳后房室束糖原大量消耗,交感神经和迷走神经兴奋性减弱,毛细血管可能受损。
OBJECTIVE: To investigate the histochemical changes of atrioventricular bundle in rats by exhaustive swimming test and to explore the influence of exhaustive training on cardiac conduction system. Methods: After exhaustive swimming, the atrioventricular bundle was taken for glycogen, lactate dehydrogenase, succinate dehydrogenase, monoamine oxidase, acetylcholinesterase and alkaline phosphatase staining respectively. The OD value of histochemical reaction was determined by image analysis system value. Results: Compared with the control group, the content of glycogen in the atrophic chamber decreased, the activity of lactate dehydrogenase increased, the activities of succinate dehydrogenase, monoamine oxidase, acetylcholinesterase and alkaline phosphatase decreased Compared with one exhaustive group, the content of glycogen in the atrophic chamber increased after one week of exhaustive swimming, the activities of lactate dehydrogenase, succinate dehydrogenase and monoamine oxidase decreased, and the activity of acetylcholinesterase and alkaline phosphatase increased . The results suggest that a large amount of glycogen in the atrioventricular bundle is consumed after an exhaustive swimming, the excitability of the sympathetic and vagal nerves may be weakened, and the capillaries may be impaired.