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目的:研究1,25二羟基维生素D3[1,25(OH)2D3]与γ射线对乳腺癌细胞株MCF-7生长及凋亡的联合作用及其机制。方法:本研究分对照组、辐射组、1,25(OH)2D3组及联合作用组。活细胞计数测定细胞存活率,克隆形成法检测细胞抑制率,TUNEL法计数凋亡细胞,流式细胞仪测定细胞周期。结果:辐射组、1,25(OH)2D3组及联合作用组的细胞存活率分别为59.8%,62.3%和11.5%,联合作用组下降最为显著(P<0.01);细胞克隆形成抑制率分别为21%、29%和63%,联合作用组高于任何一个单因素处理组(P<0.05);TUNEL检测结果显示,与对照组相比,辐射组细胞凋亡率没有显著增加(P>0.05),而联合作用组显著高于任何一个单一因素作用组(P<0.01);流式细胞仪检测显示,1,25(OH)2D3和辐射联合作用后,MCF-7细胞在G1期和G2都出现阻滞,S期延迟。结论:1,25(OH)2D3增加γ射线对乳腺癌细胞的杀伤能力。
Objective: To investigate the combined effect of 1,25 dihydroxyvitamin D3 [1,25 (OH) 2D3] and γ-rays on the growth and apoptosis of breast cancer cell line MCF-7 and its mechanism. Methods: The study divided into control group, radiation group, 1,25 (OH) 2D3 group and combined action group. Cell viability was measured by viable cell count, cell inhibition by clone formation assay, apoptotic cells by TUNEL method, and cell cycle by flow cytometry. Results: The cell viability in radiotherapy group, 1,25 (OH) 2D3 group and combined treatment group were 59.8%, 62.3% and 11.5%, respectively, and the combination group had the most significant decrease (P <0.01). The inhibition rates of cell clone formation (21%, 29% and 63% respectively), and the combined effect was higher than that of any single factor treatment group (P <0.05). The results of TUNEL assay showed that the apoptosis rate of the irradiated group was not significantly increased compared with the control group (P> 0.05), while the combined effect group was significantly higher than any single factor group (P <0.01). Flow cytometry showed that the combination of 1,25 (OH) 2D3 and radiation, MCF-7 cells in G1 and G2 are blocked, S-phase delay. Conclusion: 1,25 (OH) 2D3 increases the cytotoxicity of γ-rays to breast cancer cells.