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为了评价和管理干旱和半干旱地区的植物生长,需要了解荒漠土壤资源空间分布和土壤微生物功能.本试验在乌海、磴口和阿拉善采取蒙古沙冬青(Ammopiptanthus mongolicus)根围土壤样品,采用磷脂脂肪酸(PLFA)法结合Sherlock微生物鉴定系统,研究了蒙古沙冬青根围土壤微生物群落空间分布特征.结果表明,蒙古沙冬青根围土壤微生物PLFA有较高的多样性,3样地土壤共检测到41、31和48种磷脂脂肪酸,土壤优势PLFA为16:0、16:0 10-methy1、18:1ω9c和16:1ω7c,均以16:0(表征细菌)含量最大,16:0 10-methy1、18:1ω9c和16:1ω7c在各样地含量有所差异.蒙古沙冬青根围土壤微生物群落结构有明显空间异质性:土壤微生物以革兰氏阳性细菌(G+)为主,AM真菌、革兰氏阳性菌、革兰氏阴性菌(G-)和真菌均表现为阿拉善>乌海>磴口,而放线菌PLFA总含量表现为乌海>阿拉善>磴口.在土壤真菌生物量中,AM真菌所占比重最大,尤其在磴口和阿拉善AM真菌占到真菌生物量91%和92%,说明AM真菌是荒漠土壤微生物系统中重要组成成分.RDA分析表明,AM真菌、革兰氏阳性菌、革兰氏阴性菌、真菌和放线菌与土壤磷酸酶、总球囊霉素、氨氮和p H正相关.G+/G-与脲酶、有机碳和易提取球囊霉显著负相关,而真/细菌与易提取球囊霉素,脲酶和有机碳显著正相关.研究说明土壤磷酸酶、总球囊霉素、氨氮和p H是影响土壤微生物PLFA变化的重要因子.同时,土壤微生物群落变化规律可用于检测土地荒漠化和土壤退化状况.
In order to evaluate and manage the plant growth in arid and semi-arid areas, it is necessary to know the spatial distribution of desert soil resources and the function of soil microorganisms.In this study, soil samples from the rhizosphere of Ammopiptanthus mongolicus were collected from Wuhai, Qiaokou and Alashan, Phospholipid fatty acid (PLFA) method combined with Sherlock microbial identification system to study the spatial distribution characteristics of soil microbial communities in the rhizosphere of Ammopiptanthus mongolica.The results showed that there was a high diversity of PLFA in the soil around the Mongolian Ammopiptanthus mongolicus soil, To 41, 31 and 48 kinds of phospholipid fatty acids, soil PLFA 16: 0,16: 0 10-methy1,18: 1ω9c and 16: 1ω7c, 16: 0 Methy1, 18: 1ω9c and 16: 1ω7c were different in all kinds of soil.The soil microbial community structure of A. sojae had obvious spatial heterogeneity: the soil microorganisms were mainly Gram-positive bacteria (G +), AM fungi , Gram-positive bacteria, Gram-negative bacteria (G-) and fungi all showed Alashan> Wuhai> Guankou, while the total PLFA content of actinomycetes was Wuhai> Alashan> Guokou. Among fungal biomass, AM fungi accounted for the most , Especially AM fungi in Yokou and Alxa accounted for 91% and 92% of the fungal biomass, indicating that AM fungi are important components in the desert soil microbial system.RDA analysis showed that AM fungi, Gram-positive bacteria, Gram Negative bacteria, fungi and actinomycetes were positively correlated with soil phosphatase, total sarcadicin, ammonia nitrogen and p H, G + / G- was negatively correlated with urease, organic carbon and easily extractable Glomus, while true / Easy to extract the glomerucol, urease and organic carbon was significantly positive correlation.The study shows that soil phosphatase, total glomeramycin, ammonia nitrogen and p H is an important factor affecting soil microbial PLFA changes.At the same time, the variation of soil microbial community can be used to Detect land desertification and soil degradation.