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目的通过观测额尔敦-乌日勒对家兔动脉粥样硬化(atherosclerosis,AS)易损斑块模型血管内皮凝血纤溶物质纤维酶原激活物(t-PA)、纤维酶原激活物抑制物-1(PAI-1)、纤维蛋白原(Fbg)的影响,探讨其稳定斑块的作用机制。方法随机将50只家兔分为正常组10只,造模组40只,利用复合因素建立家兔AS易损斑块模型,并随机分为模型组,额尔敦-乌日勒组和辛伐他汀组,分别给予额尔敦-乌日勒(0.4 g·kg-1·d-1)和辛伐他汀(5 mg·kg-1·d-1)进行干预。24周后取材,运用ELISA和免疫组化方法检测各组家兔动脉粥样硬化易损斑块模型血清及主动脉t-PA、PAI、Fbg的含量及表达情况。结果与模型组比较,额尔敦-乌日勒组家兔血清PAI-1及Fbg水平显著降低(P<0.01),但对t-PA的影响差异无统计学意义(P>0.05);家兔主动脉内膜免疫组化结果显示,与模型组比较,额尔敦-乌日勒组主动脉PAI-1的表达显著降低(P<0.01),且表达量低于辛伐他汀组(P<0.05)。结论额尔敦-乌日勒可能通过降低血清血管内皮凝血纤溶物质PAI-1、Fbg水平和增加主动脉组织t-PA的表达PAI来发挥抗AS和稳定易损斑块的作用。
Objective To observe the effect of Erdeng-Wuzhile on vascular endothelial fibrinolysis-activator (t-PA), plasminogen activator inhibitor (VEGF) in atherosclerosis (AS) vulnerable plaque model in rabbits (PAI-1), fibrinogen (Fbg), to explore the mechanism of its stable plaque. Methods 50 rabbits were randomly divided into normal group (n = 10) and model group (n = 40). Rabbits were randomly divided into model group, Ertun-Urgel group and Xin The rats in the statin group were treated with Erden-Ujiller (0.4 g · kg-1 · d-1) and simvastatin (5 mg · kg-1 · d-1) Twenty-four weeks later, the content and expression of t-PA, PAI and Fbg in serum and aorta of atherosclerotic vulnerable plaque model rabbits in each group were detected by ELISA and immunohistochemistry. Results Compared with the model group, the serum levels of PAI-1 and Fbg in Ertuide-Ujiller group were significantly decreased (P <0.01), but the effect on t-PA was not statistically significant (P> 0.05) The results of intima immunohistochemistry of rabbit aorta showed that the expression of PAI-1 in the aorta of Erdeng-Wuzhile group was significantly lower than that of model group (P <0.01), and the expression of PAI-1 was lower than that of simvastatin group <0.05). Conclusion Ertar-Ujeller may exert its anti-AS and stable vulnerable plaque by decreasing PAI-1 and Fbg levels in serum and vascular endothelial coagulation fibrinolytic substance and increasing PAI in aorta tissues.