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目的:建立接骨七厘散质量标准。方法:采用TLC法对乳香进行鉴别;用HPLC法测定骨碎补中柚皮苷的含量,色谱柱为Agilent TC-C18(250 mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸(30∶70),柱温35℃,流速为0.8 ml.min-1,检测波长为283 nm。结果:TLC色谱斑点清晰;柚皮苷对照品在0.022～2.192μg范围内线性关系良好(r=0.999 9),平均回收率为95.51%,RSD为1.78%(n=9)。结论:本法简便、可靠、准确,可用于该制剂的质量控制。
Objective: To establish a bone mass standard seven bulk. Methods: TLC was used to identify Boswellia serrata. HPLC was used to determine the content of naringin. The column was Agilent TC-C18 (250 mm × 4.6 mm, 5 μm) and the mobile phase was methanol-0.1% phosphoric acid 30:70). The column temperature was 35 ℃, the flow rate was 0.8 ml.min-1 and the detection wavelength was 283 nm. Results: TLC chromatogram spots were clear. The naringin reference substance had good linearity (r = 0.999 9) in the range of 0.022-2.92 μg with an average recovery of 95.51% and a RSD of 1.78% (n = 9). Conclusion: This method is simple, reliable and accurate and can be used for the quality control of the preparation.