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人类端粒DNA由富含G的短片段重复序列(TTAGGG)串联组成,包括双链和3’端突出的单链两部分,不能编码蛋白质〔1-2〕。通常认为端粒酶是调控端粒DNA长度最重要的因素,端粒酶活性的降低将导致端粒长度的下降,细胞分裂停滞。端粒酶活性通常由其催化亚单位(端粒酶逆转录酶)所决定〔3-4〕,但奇怪的是,端粒双链DNA结合蛋白TRF1与TRF2,也能调控端粒酶活性,但它们并无直接联系。最近的研究显示人端粒保护蛋白1
Human telomere DNA is composed of tandem GST-rich short segment sequences (TTAGGG), including both single-stranded and double-stranded 3’-end and can not encode the protein [1-2]. It is generally accepted that telomerase is the most important factor that regulates telomere DNA length. Decreasing telomerase activity will result in decreased telomere length and cell division arrest. Telomerase activity is usually determined by its catalytic subunit (telomerase reverse transcriptase) [3-4], but it is strange that telomeric double-stranded DNA binding proteins TRF1 and TRF2 also regulate telomerase activity, But they are not directly linked. Recent studies have shown that human telomere protective protein 1