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与其它稀土离子的自身荧光相比,稀土Ce3+水合离子的荧光要强得多,并且在很宽的浓度范围内与其浓度成很好的正比关系,因此可以用来确定Ce3+离子的浓度.tRNA非常强烈地猝灭Ce3+水合离子的荧光.采用荧光滴定的方法研究了Ce3+-tRNA体系,并用Scatchard方法测量了Ce3+离子和tRNA的结合数和结合常数,结果发现:tRNA中有2组Ce3+结合位置,其结合常数分别为约108mol/L和105mol/L.向体系中加入精胺可使结合数和结合常数稍微降低.
Compared with the autofluorescence of other rare earth ions, the fluorescence of the rare earth Ce3 + hydrate ions is much stronger and has a good proportional relationship with its concentration over a wide range of concentrations and thus can be used to determine the concentration of Ce3 + ions. The tRNA quenches the fluorescence of Ce3 + hydrated ions very strongly. The Ce3 + -tRNA system was studied by fluorescence titration. The binding number and binding constant of Ce3 + ion and tRNA were measured by Scatchard method. The results showed that there are two groups of Ce3 + binding sites in tRNA with the binding constants of about 108 mol / L and 105mol / L. Adding spermine to the system slightly decreased the number of binding and the binding constant.