论文部分内容阅读
目的:探讨胎膜早破（PROM）孕妇外周血CDn 8+CDn 25+FoxP3n +调节性T细胞（Treg）表达水平对辅助性T细胞（Th）1/Th2平衡的影响。n 方法:选择2019年9月至2020年5月江苏省滨海县人民医院收治的PROM孕妇（PROM组）、正常足月妊娠孕妇（正常妊娠组）及正常未孕女性（未孕组）各30例为研究对象，取各组外周血，流式细胞仪测定CDn 8+CDn 25+FoxP3n +Treg比例；提取外周血单个核细胞（PBMCs），聚合酶链反应法（PCR）测定叉头翼状螺旋转录因子3（FoxP3） mRNA；Luminex液相芯片法测定Th1相关细胞因子干扰素-γ（IFN-γ）、白细胞介素（IL）-2及Th2相关细胞因子IL-10、IL-4水平；分析CDn 8+CDn 25+FoxP3n +Treg表达对Th1/Th2平衡的影响。n 结果:PROM组、正常妊娠组CDn 8+CDn 25+FoxP3n +Treg比例及FoxP3 mRNA表达水平低于未孕组[（0.15 ± 0.03）%和 （0.35 ± 0.09）%比（0.47 ± 0.11）%、0.89 ± 0.11和3.15 ± 0.67比3.75 ± 0.23]，PROM组CDn 8+CDn 25+FoxP3n +Treg比例及FoxP3 mRNA表达水平又低于正常妊娠组，差异均有统计学意义（n P<0.05）。PROM组、正常妊娠组IFN-γ、IL-2水平高于未孕组，IL-4水平低于未孕组，PROM组IFN-γ、IL-2水平又高于正常妊娠组，IL-4水平又低于正常妊娠组，差异均有统计学意义（n P<0.05）。PROM组孕妇外周血CDn 8+CDn 25+FoxP3n +Treg比例及FoxP3 mRNA表达水平与IFN-γ（n r = - 0.413、- 0.451，n P<0.05）、IL-2（n r = - 0.645、- 0.535，n P<0.05）呈负相关，与IL-4水平呈正相关（n r = 0.558、0.469，n P<0.05）。n 结论:PROM孕妇外周血CDn 8+CDn 25+FoxP3n +Treg比例较低，FoxP3 mRNA表达水平较低，均影响Th1/Th2免疫平衡，引起Th1免疫漂移，可能为PROM发生的相关免疫机制。n “,”Objective:To investigate the effect of CDn 8+ CDn 25+ FoxP3n + regulatory T cell (Treg) expression levels in peripheral blood of pregnant women with premature rupture of fetal membranes(PROM) on immune function of helper T cells (Th) 1/Th2.n Methods:Thirty cases of pregnant women with PROM (PROM group), 30 cases of normal pregnant women (normal pregnancy group) and 30 cases of normal non-pregnant women (non-pregnancy group) who treated in Binhai County People′s Hospital from September 2019 to May 2020 were collected. Peripheral blood of each group was collected and the proportion of CDn 8+ CDn 25+ FoxP3n + Treg was determined by flow cytometry. Peripheral blood mononuclear cells (PBMCs) were extracted and FoxP3 mRNA was determined by polymerase chain reaction (PCR). The levels of Th1-related cytokines interferon-γ (IFN-γ), interleukin (IL)-2, and Th2-related cytokines IL-10 and IL-4 were measured by Luminex liquid phase microarray. The effects of CDn 8+ CDn 25+ FoxP3n + Tregexpression on Th1/Th2 balance were analyzed.n Results:The proportion of CDn 8+ CDn 25+ FoxP3n + Tregand the expression of FoxP3 mRNA in PROM groupand normal pregnancy group were lower than those in non-pregnancy group: (0.15 ± 0.03) %, (0.35 ± 0.09) % vs. (0.47 ± 0.11) %; 0.89 ± 0.11, 3.15 ± 0.67 vs. 3.75 ± 0.23 , the proportion of CDn 8+ CDn 25+ FoxP3n + Treg and the expression of FoxP3 mRNA in PROM groupwere lower than those in the normal pregnancy group , and the differences were statistically significant (n P<0.05). The levels of Th1-related cytokines IFN-γ and IL-2 in PROM group and normal pregnancy group were higher than those in non-pregnancy group, the level of Th2-related cytokines IL-4 was lower than that in non-pregnancy group , the levels of IFN-γ and IL-2 in PROM group were higher than those in normal pregnancy group, the level of IL-4 was lower than that in normal pregnancy group , and the differences were statistically significant (n P<0.05). In PROM group, the proportion of CDn 8+ CDn 25+ FoxP3n + Treg and the expression of FoxP3 mRNA in peripheral blood were negatively correlated with Th1-related cytokines IFN-γ (n r = - 0.413, -0.451, n P<0.05) and IL-22 (n r = -0.645, -0.535, n P<0.05), and were positively correlated with Th2-related cytokines IL-4 (n r = 0.558, 0.469, n P<0.05).n Conclusions:The proportion of CDn 8+ CDn 25+ FoxP3n + Treg in peripheral blood of pregnant women with PROM is lower, and the expression level of related FoxP3 mRNA is lower, which all affecte the Th1/Th2 immune balance and cause Th1 immune drift, which may be the related immune mechanism of PROM.n