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目的探讨芹菜对雄性小鼠精子质量的影响,为进一步开发利用芹菜提供基础依据。方法80只SPF级昆明种健康成年雄性小鼠,按体重随机分成4个实验组:对照组、低剂量组、中剂量组、高剂量组,每天定时给对照组0.3ml生理盐水灌胃,剂量组以0.3ml不同浓度的芹菜汁灌胃,连续灌胃7d。①灌胃结束,每个实验组随机抽取10只小鼠脱臼处死,取材,应用CASA系统测定精子密度、活率及运动参数;②将剩余雄鼠恢复14d后处死,检测其精子运动参数。结果实验研究中发现,灌胃7d中剂量组小鼠精子密度增加,d级精子比例增高,精子活力和精子活率大幅度降低。灌胃恢复14d后各剂量组精子密度、精子活力及活率比较均无统计学差异。结论芹菜汁对小鼠精子的运动能力有一定影响,但其影响具有可恢复性。
Objective To investigate the effect of celery on sperm quality of male mice and provide basis for further development and utilization of celery. Methods Eighty SPF Kunming healthy adult male mice were randomly divided into four experimental groups according to body weight: control group, low-dose group, middle-dose group, and high-dose group. Each day, the control group was given 0.3 ml of normal saline. The rats were intragastrically administered with 0.3 ml different concentrations of celery juice for 7 days. 1 At the end of gavage, 10 mice in each experimental group were randomly selected and sacrificed to obtain the materials. The sperm density, survival rate and exercise parameters were measured using the CASA system. 2 The remaining male rats were sacrificed after 14 days and their sperm motility parameters were measured. RESULTS: In the experimental study, it was found that the sperm density of the mice in the middle-dose group at the 7-day intragastric administration was increased, the proportion of d-grade spermatozoa was increased, and the sperm motility and sperm motility were greatly reduced. After 14 days of gavage recovery, sperm density, sperm motility, and viability were not significantly different in each dose group. Conclusion The celery juice has a certain effect on the sperm motility of mice, but its effect is recoverable.