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目的研究姜黄素对人肝癌Ｂｅｌ７４０２细胞杀伤动力学及周期时相的影响。方法以集落形成法测定药物对增殖期与坪期细胞杀伤的量效关系曲线，以流式细胞仪及ＡＯ／ＥＢ荧光染色法分析药物对Ｂｅｌ７４０２细胞周期时相及凋亡的影响。结果姜黄素１～１０μｇ／ｍｌ作用２ｈ对不同增殖状态的Ｂｅｌ７４０２细胞均呈单指数型杀伤，但对增殖期细胞较敏感；１～１０μｇ／ｍｌ作用２４ｈ，Ｇ０－Ｇ１期细胞及Ｇ２－Ｍ期细胞的比例呈剂量依赖性减少，Ｓ期细胞及凋亡细胞的比例呈剂量依赖性增加。结论姜黄素属于优势杀灭增殖期细胞的周期时相非特异抗癌药，诱导肿瘤细胞凋亡是其重要的作用方式。
Objective To study the effects of curcumin on the killing kinetics and the phase of human hepatoma Bel7402 cells. Methods The dose-response curve of drug to cell killing in the proliferative phase and the plateau phase was determined by colony-forming method. The effect of the drug on the phase and apoptosis of Bel7402 cells was analyzed by flow cytometry and AO / EB fluorescent staining. Results Curcumin at 1 ~ 10μg / ml for 2h exhibited single exponential cytotoxicity against Bel7402 cells, but it was more sensitive to proliferating cells. After treated with 1 ~ 10μg / ml for 24 hours, cells in G0-G1 phase and G2- The proportion of cells in a dose-dependent manner, S phase cells and apoptotic cells in a dose-dependent increase. Conclusion Curcumin is an important non-specific anti-cancer drug that can kill cells in proliferative phase and induce apoptosis of tumor cells.