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目的探讨内毒素在大鼠肝硬化发生发展中的作用。方法采用饮用０．０３％硫代乙酰胺（ＴＡＡ）四个月复制伴有肠源性内毒素血症的肝硬化大鼠模型，观察ＴＡＡ与ＴＡＡ＋内毒素（ＬＰＳ）对肝组织胶原蛋白含量的影响，并测定血浆与肝组织匀浆的肿瘤坏死因子α （ＴＮＦ α）、内皮素（ＥＴ－１）、一氧化氮（ＮＯ）与丙二醛（ＭＤＡ）含量。结果 ＴＡＡ组与ＴＡＡ＋ＬＰＳ组大鼠血浆与肝组织的ＴＮＦ α、 ＥＴ－１、 ＮＯ、 ＭＤＡ与肝组织胶原蛋白含量均明显高于正常对照组。结论内毒素可加剧肝纤维增生并发展为肝硬化。
Objective To investigate the role of endotoxin in the development of cirrhosis in rats. Methods Rat liver cirrhosis rat models with intestinal endotoxemia were replicated in four months with 0.03% thioacetamide (TAA) drinking. The effects of TAA and TAA + endotoxin (LPS) on the hepatic collagen content The levels of tumor necrosis factor α (TNF α), endothelin (ET-1), nitric oxide (NO) and malondialdehyde (MDA) in plasma and liver homogenates were measured. Results The levels of TNFα, ET-1, NO, MDA and liver collagen in TAA group and TAA + LPS group were significantly higher than those in normal control group. Conclusion Endotoxin can aggravate hepatic fibrosis and develop cirrhosis.