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目的 为了筛选抗乙型肝炎病毒(HBV)药物的细胞模型,本研究探索了转基因细胞2.2.15的培养条件及分泌表达的动力学变化。方法 用ELISA法动态检测2.2.15细胞分泌表达HBsAg和HBeAg的动力学变化。用PCR技术检测细胞和细胞培养上清中HBV-DNA。结果 2.2.15细胞在适宜的培养条件下能够持续分泌表达HBsAg和HBeAg,培养第6天达分泌高峰,第7天有所下降。PCR结果证明细胞和培养液上清中HBV-DNA为阳性。结论 转基因细胞2.2.15可作为筛选抗HBV药物的细胞模型。
Objective To screen cell models of anti-hepatitis B virus (HBV) drugs, this study explored the culture conditions and kinetic changes of secreted and expressed 2.2.15 cells. Methods The kinetic changes of secreted HBsAg and HBeAg in 2.2.15 cells were detected by ELISA. Detection of HBV-DNA in cells and cell culture supernatants by PCR. Results 2.2.15 cells under continuous culture in the appropriate conditions for the expression of HBsAg and HBeAg secretion peaked on the 6th day of culture, decreased on the 7th day. PCR results showed that HBV-DNA was positive in cells and culture supernatants. Conclusion Transgenic cells 2.2.15 can be used as a cell model for screening anti-HBV drugs.