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目的 :了解内蒙古地区胰腺癌患者P5 3抑癌基因的突变情况。方法 :采用不对称PCR—SSCP技术检测胰腺癌患者P5 3基因在第 5 ,6 ,7和 8外显子的基因突变 ,它与传统的PCR—SSCP相比有更多的优势 ,克服了传统方法中双链DNA不完全变性或非特异扩增造成的解读困难和错误判读 ,而且应用一对引物中不同当量的 2个引物配成的反应混合物Mix1和Mix2 ,对标本进行两次检验 ,可以两次判定结果 ,进一步增加了实验的可靠性。结果 :本实验扩增出的 2 1例石蜡标本中有 5例检出了突变 ,突变率为 2 3 8%。结论 :提示在内蒙古地区 ,胰腺癌的发生与P5 3抑癌基因的突变相关。
Objective: To understand the mutation of P53 tumor suppressor gene in patients with pancreatic cancer in Inner Mongolia. METHODS: Asymmetric PCR-SSCP was used to detect gene mutations in the 5th, 6th, 7th, and 8th exons of P53 gene in patients with pancreatic cancer. It has more advantages than traditional PCR-SSCP, which overcomes the traditional In the method, double-stranded DNA is incompletely denatured or non-specifically amplified, and the interpretation and misinterpretation are caused. Moreover, the reaction mixtures Mix1 and Mix2 formulated with two primers of different equivalent amounts in a pair of primers are used to perform double-test on the specimen. The results of the two determinations further increase the reliability of the experiment. RESULTS: Among the 21 paraffin specimens amplified in this experiment, 5 mutations were detected and the mutation rate was 238%. Conclusion : It is suggested that the occurrence of pancreatic cancer is related to the mutation of P53 tumor suppressor gene in Inner Mongolia.