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目的探讨Galectin-3和β-catenin在结直肠腺癌组织中的表达及其与临床病理参数的关系。方法应用微波-EliVisionTM(MW-EliVisionTM)免疫组织化学染色技术检测60例结直肠腺癌、30例腺瘤及30例正常黏膜组织(远离癌组织4cm以上)中Galectin-3和β-catenin的表达,分析表达情况及其与结直肠腺癌临床病理参数的关系。结果 Galectin-3和β-catenin蛋白在正常黏膜组织、结直肠腺瘤及腺癌中的阳性表达逐渐增强,差异均有统计学意义(P<0.05)。二者在TNM分期、浸润程度、淋巴结转移等参数中差异有统计学意义(P<0.05)。且在不同分化程度之间差异有统计学意义(P<0.05)。二者在结直肠腺癌组织中阳性表达呈正相关(r=0.696,P<0.01)。结论 Galectin-3和β-catenin高表达与结直肠腺癌高侵袭能力、淋巴结转移有一定相关性,可作为潜在预测大肠癌浸润、转移的敏感指标。
Objective To investigate the expression of Galectin-3 and β-catenin in colorectal adenocarcinoma and its relationship with clinicopathological parameters. Methods The expressions of Galectin-3 and β-catenin in 60 cases of colorectal adenocarcinoma, 30 cases of adenoma and 30 cases of normal mucosa (more than 4 cm away from cancer) were detected by MW-EliVisionTM immunohistochemical staining , Analyze the expression and its relationship with clinicopathological parameters of colorectal adenocarcinoma. Results The positive expression of Galectin-3 and β-catenin protein in normal mucosa, colorectal adenoma and adenocarcinoma gradually increased, the difference was statistically significant (P <0.05). There was a significant difference in TNM stage, infiltration degree and lymph node metastasis (P <0.05). There was significant difference between different degree of differentiation (P <0.05). There was a positive correlation between them in colorectal adenocarcinoma (r = 0.696, P <0.01). Conclusion The overexpression of Galectin-3 and β-catenin may be related to the high invasiveness and lymph node metastasis of colorectal adenocarcinoma, which may be used as a sensitive index to predict the invasion and metastasis of colorectal cancer.