小决明(Cassia tora)的种子入中药,具有明目、抗疲劳、利尿等功效。本实验探讨了其甲醇提取物及其成分的抗突变活性。 将粉碎的小决明种子,以甲醇回流提取,得甲醇提取物。将干燥的甲醇提取物依次以二氯甲烷、正丁醇和水萃取,分得相应的提取部分。将二氯甲烷部分以硅胶柱层析,用二氯甲烷-甲醇梯度洗脱,得大黄酚、黄决明素和 The seeds of Cassia tora are introduced into traditional Chinese medicine and have functions such as eyesight, anti-fatigue and diuresis. This experiment explored the antimutagenic activity of its methanol extract and its components. The crushed Cassia cassia seed was extracted by refluxing with methanol to obtain a methanol extract. The dried methanol extract was sequentially extracted with dichloromethane, n-butanol and water, and the corresponding fractions were obtained. The dichloromethane fraction was chromatographed on a silica gel column eluting with a dichloromethane-methanol gradient to give chrysophanol, flavozein and