An In Vitro Biological Model for Evaluation of the Anti-Dandruff Performance of Hair Care Products

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An in vitro biological model for evaluation of the anti-dandruff performance of hair care products was established. In this model, Malasseziafurfur(M.furfur) was used as indicator bacteria with pigskin as substrate. This model functions in following steps: pretreatment for the substrate; inoculation of indicator bacteria; measuring the bacteriostatic efficacy by the contact plates after washing the pigskin with the sample shampoo; calculation of the effective index P; evaluation of the anti-dandruff performance by the effective index P. Three kinds of anti-dandruff agents including BPT,ZPT and OCT were tested by the model. Results showed that the order for antibacterial efficacy of the anti-dandruff agents within the mass fraction range of 0.1%~2.0% was BPT>ZPT>OCT. Results of the evaluation on each type of shampoo showed that: one displays no anti-dandruff efficacy; three displays medium anti-dandruff efficacy; two displays strong anti-dandruff efficacy.
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