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1994年7月13日～8月27日，甘肃省积石山县居集乡劳动村，发生一起不明原因的流行性腹泻。为了探讨病因，有效地控制腹泻病流行。我们采用BA－ELISA（生物素──酶联免疫吸附）法测定了病人血清中宋内氏志贺氏菌IgA抗体，结果11价病人血清全部阳性，抗体滴度在1：32～1：1024之间。同时从1例未用药患者粪便中分离出1株宋内氏志贺氏菌。经血清学和病原学检测分析，这次腹泻病流行是由宋内氏志贺氏菌引起的。
July 13, 1994 to August 27, Jishishan, Gansu Province, Jixi Township Labor Village, an unknown cause of epidemic diarrhea. In order to explore the cause, effectively control the epidemic of diarrhea. We determined the IgA antibody against Shigella sonnei in the serum of patients by BA-ELISA (biotin-ELISA). The results showed that the serums of 11 patients were all positive, and the antibody titer was 1:32 to 1: 1024 between. At the same time, one strain of Shigella sonnei was isolated from the stool of one patient who did not receive drugs. Serological and pathogenic detection analysis, the epidemic of diarrhea is caused by Shigella sonnei.