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本文就硝化煤尘提取物在有或无微粒体酶激活的条件下,对病毒在哺乳动物细胞中干扰素诱导的影响并结合其致突变作用进行了研究。干扰素诱导和测试使用AO/PR/8/34流感和副流感病毒和Rhesus猴肾(LLC-MK_2)和人Chang结缔组织(1-5C-4克隆)细胞系。按Ames方法用雄性Wistar/Lewis大鼠制备S_9。根据Whong介绍的方法进行煤尘提取和硝化。硝化煤尘提取物与马血清混合液及在次相应的柱层析分离物都分别进行病毒干扰素诱导测试及致突变试验。煤尘及其溶解性提取物、硝化
In this paper, the influence of nitrocellulose dust extracts on the induction of interferon in mammalian cells with or without microsomal enzyme activation and its mutagenesis were investigated. Interferon induction and testing used AO/PR/8/34 influenza and parainfluenza virus and Rhesus monkey kidney (LLC-MK_2) and human Chang connective tissue (1-5C-4 clone) cell lines. S_9 was prepared using male Wistar/Lewis rats according to the Ames method. Coal dust extraction and nitrification were carried out according to the method described by Whong. Both the nitrified coal dust extract and horse serum mixture and the corresponding column chromatographic separations were tested for viral interferon induction and mutagenesis, respectively. Coal dust and its soluble extract, nitrification