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为了缓解煤炭开采对神东矿区农业生产和地表环境造成的破坏,试验模拟神东矿区干旱缺水特点,以煤炭开采塌陷区退化土壤为供试基质,玉米为供试植物,研究丛枝菌根真菌(每克干土中含63个孢子)和不同浓度的外源钙(5,10,20,40,80mmol/L)协同效应对中度干旱胁迫下苗期玉米生长的影响。结果表明,干旱胁迫下,20mmol/L外源钙与丛枝菌根真菌协同效应最优,玉米质量平均每株可达4.03g,组织含水率和叶色值分别达到91.68%和43.67,玉米植株内氮磷钾累积量显著高于其他处理;同时,玉米根际土壤球囊霉素相关蛋白增加最为明显,总球囊霉素和易提取球囊霉素分别达到4.3和1.6mg/g,根际土壤中有机质含量增加明显。丛枝菌根真菌与外源钙联合作用有利于玉米的生长,缓解了干旱胁迫对苗期玉米生长的影响,接种丛枝菌根真菌对矿区退化土壤具有显著改良效应。
In order to alleviate the damage caused by coal mining to agricultural production and surface environment in Shendong Mining Area, the experiment simulated the characteristics of drought and water shortage in Shendong Mining Area. The deteriorated soil in subsidence area of coal mining was taken as the test substrate and corn was used as the test plant to study arbuscular mycorrhizal fungi Effects of synergistic effects of fungi (63 spores per gram of dry soil) and different concentrations of exogenous calcium (5, 10, 20, 40, 80mmol / L) on growth of maize seedlings under moderate drought stress. The results showed that under the drought stress, the synergistic effect of 20mmol / L exogenous calcium and arbuscular mycorrhizal fungi was the best. The average quality of corn was 4.03g per plant and the moisture content and leaf color value were 91.68% and 43.67 respectively. The contents of NPK accumulation in rhizosphere soil of maize increased most significantly at the same time, the total amount of glomycin and glibenclamide was up to 4.3 and 1.6 mg / g, respectively Soil organic matter content increased significantly. The combination of arbuscular mycorrhizal fungi and exogenous calcium was beneficial to the growth of maize, alleviating the effect of drought stress on the growth of maize at seedling stage, and arbuscular mycorrhizal fungi inoculation significantly improved the degraded soil in the mining area.