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目的对比分析非小细胞肺癌纤支镜活检与相应手术标本的ＤＮＡ含量，以评价非小细胞肺癌纤支镜活检ＤＮＡ含量分析的可靠性。方法应用ＣＡＳ—２００型图像细胞仪对２２例非小细胞肺癌纤支镜活检标本和相应手术标本进行ＤＮＡ含量分析和比较研究。结果纤支镜组与手术组标本异倍体符合率为９１％，但手术组ＤＮＡ指数明显高于纤支镜组（Ｐ＜００５）。结论纤支镜活检标本的ＤＮＡ含量分析与手术标本一样可靠，ＤＮＡ倍体水平应是纤支镜活检ＤＮＡ含量分析的重要指标，不足之处为纤支镜活检的ＤＮＡ指数不如手术标本准确。
Objective To compare the DNA content of bronchoscopic biopsies and corresponding surgical specimens of non-small cell lung cancer (NSCLC) for the purpose of evaluating the reliability of DNA microanalysis of non-small cell lung cancer. Methods DNA content analysis and comparative study of 22 specimens of non-small cell lung cancer biopsy specimens and corresponding surgical specimens were performed using a CAS-200 image cytometer. Results The coincidence rate of aneuploidy in the bronchoscopic group and the surgical group was 91%, but the DNA index in the surgical group was significantly higher than that in the bronchoscopic group (P < 0.05). Conclusions DNA content analysis of bronchoscopic biopsy specimens is as reliable as surgical specimens. DNA ploidy levels should be an important indicator for the analysis of DNA content in bronchoscopic biopsies. The disadvantage is that the DNA index of bronchoscopic biopsies is not as accurate as the surgical specimens.